Invitrogen™ SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag Recombinant Protein

Description

Recombinant Protein

Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

Specifications

• For Use With (Application): Control, ELISA, Functional Assay, Surface Plasmon Resonance, Western Blot
• Formulation: PBS with no preservative; pH 7.4
• Molecular Weight (g/mol): 103 kDa
• Name: SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag
• Quantity: 100 μg
• Source: HEK293 cells
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein S1
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 (at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Spike S1, the ED50 of SARS-COV-2 Spike S1 is 6.79 ng/mL Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: Fc-tag, His-tag
• Sequence: SARS-CoV-2 Spike Protein S1, amino acids Val11-Arg682 (Accession # YP_009724390.1) with a C-terminal human Fc-tag and His-tag
• Content And Storage: -20°Cor to +80°C if preferred
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% by SDS-PAGE