Invitrogen™ SARS-CoV-2 Spike Protein S1 (mutant) His-tag Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. In general, recombinant proteins are provided as lyophilized powder.

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.

Specifications

• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Molecular Weight (g/mol): 93.5 kDa
• Name: SARS-CoV-2 Spike Protein S1 (mutant) His-tag
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV2; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike Protein S1
• Biological Activity: Immobilized human ACE2 protein (mFc tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag), the EC50 of SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag) is 35-200 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Met1-Arg685
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized human ACE2 protein (mFc tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag), the EC50 of SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag) is 35-200 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE.