Invitrogen™ SARS-CoV-2 Spike Protein S1/S2 (B.1.1.7) His-tag, trimer Recombinant Protein

Description

Recombinant Protein

It is recommended that the protein be aliquoted for optimal storage. It is recommended that sterile water (400 μL) be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV. SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with (69-70, 145) deletions and N501Y, A570D, D614G, T716I, F817P, A892P, A899P, A942P, S982A, K986P, V987P, D1118H substitutions and furin cleavage site mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag. The mutations were identified in the SARS-CoV-2 varient known as B.1.1.7.

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Specifications

• For Use With (Application): ELISA
• Format: Lyophilized
• Formulation: PBS with 0.01% mannitol, 0.01% Tween 80, 5-8% trehalose and no preservative; pH 7.4
• Molecular Weight (g/mol): 136.18 kDa
• Name: SARS-CoV-2 Spike Protein S1/S2 (B0.1.1.7) His-tag, trimer
• Purification Method: SDS-PAGE
• Quantity: 100 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <1.0 EU/μg
• Gene Alias: Coronavirus; COVID; Covid 19; COVID19; COVID-19; SARS; SARS Coronavirus; SARS CoV; SARS CoV2; SARS CoV-2; SARS Spike Protein; SARS-CoV-2
• Common Name: SARS-CoV-2 Spike (trimer) Protein
• Biological Activity: Immobilized human ACE2 protein (mFc tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B0.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) ,the EC50 of SARS-CoV-2 B0.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) is 5-30 ng/mL)
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with variant B0.1.1.7 mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag. Full sequence information available in Product Specfic Information section.
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Activity: Immobilized human ACE2 protein (mFc tag) (at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B0.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) ,the EC50 of SARS-CoV-2 B0.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag) is 5-30 ng/mL)
• Expression System: HEK293 cells
• Form: Lyophilized
• Purity or Quality Grade: >90% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.