Invitrogen™ SARS-CoV/SARS-CoV-2 NP Monoclonal Antibody (3864)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA547524

Catalog No. PIMA547524

Description

Specificity: Specific for the nucleoprotein of the SARS virus (IFA & ELISA) and COVID-19 virus (ELISA). Negative for human coronaviruses 229E & OC43. Reactive with the nucleocapsids of Variants: UK B.1.1.7, SA B.1.351, Brazil P.1 & B.1.617.2. Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography. The final preparation is formulated to a protein concentration of 100 μg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 mL. This product contains no stabilizing proteins and should be stored at 4C until ready for use. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200. for ELISA.

Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. SARS-CoV-2 Nucleocapsid Protein is associated with nucleic acid. It is the most abundant protein for coronavirus. Because of the strong immunogenicity of coronavirus Nucleocapsid, it is believed that SARS-CoV-2 Nucleocapsid Protein has potential value for the diagnosis of the virus.

Specifications

• Antigen: SARS-CoV/SARS-CoV-2 NP
• Classification: Monoclonal
• Concentration: 100 μg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Virus
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunocytochemistry
• Clone: 3864
• Conjugate: Unconjugated
• Gene Alias: nucleoprotein
• Immunogen: Proprietary sequence.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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