Sartorius Sartobind Lab Affinity IDA75

Description

Ready-to-use membrane adsorbers for immobilized metal affinity chromatography (IMAC) in syringe filter format. Superior flow rates and high binding capacity based on the patented macroporous Sartobind™ membranes with iminodiacetic acid (IDA).

• Based on the Patented Sartobind™ Platform Sartobind™ Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format.
• They are based on the patented macroporous Sartobind™ membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity.
• The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein.
• Ultimate Versatility Sartobind™ Lab IDA units are available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit.
• Equipped with standard Luer lock connectors, Sartobind™ Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications.
• The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.
• Fewer preparative steps
• Greatly reduced cycle times
• Achieve higher yields in less time
• Purify equipment-free or with an FPLC system

Specifications

• Bed Volume: 2.1 mL
• Dead Volume: 1.3 mL
• Disposable: Reusable
• Filter Type: Air Syringe Filter
• For Use With (Equipment): Syringe
• Material: Polyester
• Material (Membrane): Cellulose
• Pore Size: 5 μm
• Sterility: Non-sterile
• For Use With (Application): His-tagged Protein Purification
• Max. Pressure: 0.6 mPa
• Membrane Type: Metal Chelate
• Type: Membrane Adsorber
• Volume (Metric): 2.1 mL

Safety and Handling

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