MilliporeSigma™ SCAP, Mouse, Unlabeled, Clone: 9D5,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1249

Catalog No. MABS1249MI

Description

Sterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP mediates the activation of membrane-bound transcription factors SREBPs (sterol-regulatory element binding proteins) for transcribing genes encoding cholesterol biosynthetic enzymes. In sterol-deprived cells, SCAP escorts SREBPs from ER to Golgi, where active fragments of SREBPs are released from membrane by proteolysis. Sterol accumulation inuduces a negative regulatory mechanism, where SCAP becomes bound by Insigs and trapped in the ER, preventing further delivery of SREBPs to the Golgi for proteolytic release. SCAP spans the ER membrane eight times (a.a. 19-39, 280-300, 313-333, 345-365, 402-422, 424-444, 519-539, 709-729) with 4 lumenal and 5 cytoplasmic regions, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-18 and 730-1276). The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2–6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. Mutations within the sterol-sensing region disrupt Insig binding and prevent sterol-mediated ER retention of SCAP-SREBP.

Specifications

• Antigen: SCAP
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P97260
• Immunogen: His-tagged recombinant fragment corresponding to the fourth lumenal domain of hamster SCAP.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001230965
• Target Species: Hamster
• Form: Purified

• Applications: Western Blot
• Clone: 9D5
• Conjugate: Unconjugated
• Gene: SCAP
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 9D5 detected the target band in lysate and membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cell line.
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.