Invitrogen™ SCN10A Monoclonal Antibody (S134)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527663

Catalog No. PIMA527663

Description

1 μg/mL of MA5-27663 was sufficient for detection of Nav1.8 in 10 μg of COS cell lysate transiently expressing Nav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 220kDa. No cross reactivity against other Nav channels.

Scn10a is a tetrodotoxin-resistant voltage-gated sodium channel alpha subunit. Epithelial sodium channels are amiloride-sensitive members of the Degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Scn10a mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Scn10a forms a sodium-selective channel through which sodium ions may pass in accordance with their electrochemical gradient. Scn10a also plays a role in neuropathic pain mechanisms. Structurally, members of Deg/ENaC superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddels syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1). Diseases associated with SCN10A include Episodic Pain Syndrome, Familial, 2 and Sodium Channelopathy-Related Small Fiber Neuropathy.

Specifications

• Antigen: SCN10A
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: Q62968, Q6QIY3, Q9Y5Y9
• Gene Symbols: Scn10a
• Immunogen: Fusion protein amino acids 1724-1956 of rat Nav1.8.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry, Microarray
• Clone: S134
• Conjugate: Unconjugated
• Gene: Scn10a
• Gene Alias: FEPS2; hPN3; mPN3; Na(V)1.8; Nav1.8; peripheral nerve sodium channel 3; PN3; Scn10a; Sensory neuron sodium channel; SNS; Sodium channel protein type 10 subunit alpha; sodium channel protein type X subunit alpha; sodium channel type X alpha polypeptide; sodium channel voltage-gated type X alpha polypeptide; sodium channel, voltage gated, type X alpha subunit; sodium channel, voltage-gated, type 10, alpha polypeptide; sodium channel, voltage-gated, type X, alpha; sodium channel, voltage-gated, type X, alpha polypeptide; sodium channel, voltage-gated, type X, alpha subunit; sodium voltage-gated channel alpha subunit 10; voltage gated sodium channel; voltage-gated sodium channel subunit alpha Nav1.8
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 20264, 29571, 6336
• Content And Storage: -20°C
• Form: Liquid