SCN5A Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5111903

Catalog No. PIPA5111903

Description

Reconstitute antibody with 50 μL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Homology: Rat - 36/39 amino acid residues identical; mouse - 34/39 amino acid residues identical.

Scn5a is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. Scn5a is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in Scn5a are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms of Scn5a. Voltage-gated sodium channesl (VGSCs) mediate regenerative cell membrane depolarization and conduction of electrical signalling in nerves and muscles. Expression of Scn5a is also detected in lymphocytes, glia, and fibroblasts. Further, Scn5a mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, Scn5a forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. Functionally, Scn5a is responsible for the initial upstroke of the action potential and the channel inactivation is regulated by intracellular calcium levels. Voltage-gated sodium channels (NaV) are responsible for action potential initiation and propagation in excitable cells, including nerve, muscle, and neuroendocrine cell types. Scn5a are also expressed at low levels in non-excitable cells, where their physiological role is unclear. Diseases associated with SCN5A include Long Qt Syndrome-3 and Brugada Syndrome 1.

Specifications

• Antigen: SCN5A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SCN5A
• Gene Alias: CDCD2; CDCD2VF1; CIN5; CMD1E; CMPD2; HB1; HB2; HBBD; HH1; HH1CMD1E; ICCD; IVF; LQT3; LQT3SSS1CMPD2; mH1; Nav1.5; Nav1.5c; PFHB1; RATRSKM2X; RH1; RSkM2; RSKM2X; SCAL; Scn2x; SCN5A; SkM1; SkM2; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type 5 subunit alpha; Sodium channel protein type V subunit alpha; SSS1; VF1; Voltage-gated sodium channel subunit alpha Nav1.5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25665, 6331
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.95 mg/mL
• Formulation: PBS with 1% BSA and 0.05% sodium azide; pH 7.4
• Gene Accession No.: P15389, Q14524
• Gene Symbols: SCN5A
• Immunogen: GSTfusion protein with amino acid residues 1978-2016 of human NaV1.5 (Intracellular, C-terminus)
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG