Invitrogen™ SCNN1B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA577817

Catalog No. PIPA577817

Description

1 μg/mL of PA5-77817 was sufficient for detection of beta-ENaC in 20 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. Detects approximately 87kDa.

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. Defects in SCNN1B are a cause of autosomal recessive pseudohypoaldosteronism type 1 (PHA1) [MIM:264350]. PHA1 is a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal recessive form that is severe, and the dominant form which is more milder and due to defects in mineralocorticoid receptor. Autosomal recessive PHA1 is characterized by an often fulminant presentation in the neonatal period with dehydration, hyponatraemia, hyperkalaemia, metabolic acidosis, failure to thrive and weight loss. Defects in SCNN1B are a cause of Liddle syndrome. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.

Specifications

• Antigen: SCNN1B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SCNN1B
• Gene Alias: Amiloride-sensitive sodium channel subunit beta; amiloride-sensitive sodium channel subunit beta 1; amiloride-sensitive sodium channel subunit beta-like protein; BESC1; Beta ENaC; beta-ENaC; Beta-NaCH; betaxENaC; ENaC beta; ENaCb; enacbeta; epithelial Na(+) channel subunit beta; epithelial sodium channel beta-2 subunit; epithelial sodium channel beta-3 subunit; epithelial sodium channel, nonvoltage-gated 1, beta; nasal epithelial sodium channel beta subunit; nonvoltage-gated sodium channel 1 subunit beta; RNENACB; SCNEB; SCNN 1B; SCNN1B; scnn1b.L; scnn1b-a; scnn1b-b; sodium channel epithelial 1 beta subunit; sodium channel, non voltage gated 1 beta subunit; sodium channel, non voltage gated 1 beta subunit L homeolog; sodium channel, nonvoltage-gated 1 beta; sodium channel, nonvoltage-gated 1, beta; sodium channel, non-voltage-gated 1, beta subunit; sodium channel, nonvoltage-gated, type I, beta; XELAEV_18045378mg
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100754564, 20277, 24767, 378536, 6338
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Gene Accession No.: P37090, P51168, Q9WU38
• Gene Symbols: SCNN1B, scnn1b.L
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Hamster, Frog
• Product Type: Antibody
• Isotype: IgG