Invitrogen™ SDHA Monoclonal Antibody (2E3GC12FB2AE2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 459200

Catalog No. 45-920-0

Description

ICC application requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol). Positive control: Human heart mitochondria.

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29.

Specifications

• Antigen: SDHA
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: HEPES buffered saline with 0.02% sodium azide; pH 7.5
• Gene Accession No.: P31039, P31040, Q8K2B3, Q920L2
• Gene Symbols: SDHA
• Immunogen: Full length native protein (purified) corresponding to Cow SDHA. Purified mitochondrial complex II (Cow).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Western Blot, Immunocytochemistry
• Clone: 2E3GC12FB2AE2
• Conjugate: Unconjugated
• Gene: SDHA
• Gene Alias: 1500032O14Rik; 2310034D06Rik; 4921513A11; C81073; CMD1GG; Flavoprotein subunit of complex II; FP; im:7141001; mitochondrial succinate dehydrogenase complex subunit A; PGL5; SDH1; SDH2; SDHA; SDHA protein; SDHF; SDHFP1; si:dkeyp-84f11.2; succinate dehydrogenase [ubiquinone] flavoprotein subunit; succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; succinate dehydrogenase complex flavoprotein subunit A; succinate dehydrogenase complex subunit A; succinate dehydrogenase complex subunit A flavoprotein; succinate dehydrogenase complex subunit A, flavoprotein (Fp); succinate dehydrogenase complex, subunit A, flavoprotein (Fp); succinate dehydrogenase flavoprotein subunit; succinate dehydrogenase flavoprotein subunit A; zgc:56051
• Host Species: Mouse
• Purification Method: IgG fraction
• Regulatory Status: RUO
• Gene ID (Entrez): 157074, 281480, 6389, 66945
• Content And Storage: 4°C
• Form: Liquid