Invitrogen™ SEMA3F Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143760

Catalog No. PIPA5143760

Description

The antibody detects a 95 kDa* protein corresponding to the apparent molecular mass of Sema-3F on SDS-PAGE immunoblots of adult mouse brain and liver.

The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.

Specifications

• Antigen: SEMA3F
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SEMA3F
• Gene Alias: LOW QUALITY PROTEIN: semaphorin-3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3 F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; Sema III/F; Sema IV; Sema3f; Sema4; SemaIV; SEMA-IV; SEMAK; semaphorin 3F; semaphorin III/F; semaphorin IV; Semaphorin-3F
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20350, 315996, 6405
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: O88632, Q13275
• Gene Symbols: SEMA3F
• Immunogen: Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG