Semaphorin 4D/CD100 Mouse, Clone: SEMA4D/933, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247796B	Novus Biologicals NBP2477960.2MG	0.2 mg

Description

Semaphorin 4D/CD100 Monoclonal specifically detects Semaphorin 4D/CD100 in Human, Primate samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: Semaphorin 4D/CD100
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Immunology
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 10507
• Immunogen: Human recombinant SEMA4D protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: SEMA4D/933
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Primate
• Gene Alias: A8, CD100 antigen, CD100M-sema-G, coll-4, FLJ33485, FLJ34282, FLJ39737, FLJ46484, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D, MGC169138, MGC169141, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D, semaphorin-4D
• Gene Symbols: SEMA4D
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.