Cytiva Sepharose™ Fast Flow Cation Exchange Media

Catalog No.	Binding Capacity	Product Type	Packaging Quantity
45-002-934	70 mg Ribonuclease A/mL Medium	SP Sepharose Fast Flow Media	25 mL
45-002-933	70 mg Ribonuclease A/mL Medium	SP Sepharose Fast Flow Media	300 mL
45-002-931	50 mg Ribonuclease A/mL Medium	CM Sepharose Fast Flow Media	500 mL
45-002-932	50 mg Ribonuclease A/mL Medium	CM Sepharose Fast Flow Media	25 mL

Catalog No. 45-002-934 Supplier Cytiva Supplier No. 17072910

Description

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Composed of crosslinked 6% agarose beads
• Reproducible
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

SP Sepharose:

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.18 to 0.25mmol H⁺/mL medium
• Binding capacity: 70mg ribonuclease A/mL medium

CM Sepharose:

• Ligand: Carboxymethyl
• Ion Exchanger Type: Weak cation exchanger
• Ionic Capacity: 0.09 to 0.13mmol Hl⁺/mL medium
• Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications

• Quantity: 25 mL
• For Use With (Application): For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
• Storage Requirements: 4°C to 30°C
• Anion Exchange Type: Strong CatIon Exchanger
• Anion Exchange Capacity: 0.18 to 0.25 mM Hl+/mL
• Binding Capacity: 70 mg Ribonuclease A/mL Medium
• Product Type: SP Sepharose Fast Flow Media
• Flow Rate: 400 to 700 cm/Hoμr
• Particle Size: 45 to 165 μm
• Max. Pressure: 100 kPa
• Matrix: 6% Cross-Linked Agarose
• pH Range: 4 to 13
• Storage Buffer: 20% Ethanol, 0.2 M Sodium Acetate
• Ligand Type: Sulphopropyl
• Packaging Quantity: 25 mL