Cytiva Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Supplier:  Cytiva 17072910

Catalog No. 45-002-934

Description

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

• Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
• Composed of crosslinked 6% agarose beads
• Reproducible
• Part of Sepharose Fast Flow ion exchange platform
• High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
• Available in range of pre-packed process development tools

SP Sepharose:

• Ligand: Sulphopropyl
• Ion Exchanger Type: Strong cation exchanger
• Ionic Capacity: 0.18 to 0.25mmol H⁺/mL medium
• Binding capacity: 70mg ribonuclease A/mL medium

CM Sepharose:

• Ligand: Carboxymethyl
• Ion Exchanger Type: Weak cation exchanger
• Ionic Capacity: 0.09 to 0.13mmol Hl⁺/mL medium
• Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications

• Quantity: 25 mL
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
• Anion Exchange Type: Strong CatIon Exchanger
• Binding Capacity: 70 mg Ribonuclease A/mL Medium
• Flow Rate: 400 to 700 cm/Hoμr
• Max. Pressure: 100 kPa
• pH Range: 4 to 13
• Ligand Type: Sulphopropyl

• For Use With (Application): For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
• Storage Requirements: 4°C to 30°C
• Anion Exchange Capacity: 0.18 to 0.25 mM Hl+/mL
• Product Type: SP Sepharose Fast Flow Media
• Particle Size: 45 to 165 μm
• Matrix: 6% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol, 0.2 M Sodium Acetate
• Packaging Quantity: 25 mL