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  5. Cytiva Sepharose™ Fast Flow Cation Exchange Media

Cytiva Sepharose™ Fast Flow Cation Exchange Media

Catalog No. 45002934
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Binding Capacity:
50 mg Ribonuclease A/mL Medium
70 mg Ribonuclease A/mL Medium
Product Type:
CM Sepharose Fast Flow Media
SP Sepharose Fast Flow Media
Packaging Quantity:
25 mL
300 mL
500 mL

Description

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

TRUSTED_SUSTAINABILITY

Specifications

Quantity 25 mL
For Use With (Application) For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
Stability Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
Storage Requirements 4°C to 30°C
Anion Exchange Type Strong CatIon Exchanger
Anion Exchange Capacity 0.18 to 0.25 mM Hl+/mL
Binding Capacity 70 mg Ribonuclease A/mL Medium
Product Type SP Sepharose Fast Flow Media
Flow Rate 400 to 700 cm/Hoμr
Particle Size 45 to 165 μm
Max. Pressure 100 kPa
Matrix 6% Cross-Linked Agarose
pH Range 4 to 13
Storage Buffer 20% Ethanol, 0.2 M Sodium Acetate
Ligand Type Sulphopropyl
Packaging Quantity 25 mL
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