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Cytiva Sepharose™ Fast Flow Cation Exchange Media
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Binding Capacity:
50 mg Ribonuclease A/mL Medium
70 mg Ribonuclease A/mL Medium
Product Type:
CM Sepharose Fast Flow Media
SP Sepharose Fast Flow Media
Packaging Quantity:
25 mL
300 mL
500 mL
Description
Used for preparative protein separations in both research and industrial applications. Cytiva CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.
- Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
- Composed of crosslinked 6% agarose beads
- Reproducible
- Part of Sepharose Fast Flow ion exchange platform
- High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
- Available in range of pre-packed process development tools
SP Sepharose:
- Ligand: Sulphopropyl
- Ion Exchanger Type: Strong cation exchanger
- Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
- Binding capacity: 70mg ribonuclease A/mL medium
- Ligand: Carboxymethyl
- Ion Exchanger Type: Weak cation exchanger
- Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
- Binding capacity: 50mg ribonuclease A/mL medium
Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications
Specifications
Quantity | 25 mL |
For Use With (Application) | For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals |
Stability | Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol |
Storage Requirements | 4°C to 30°C |
Anion Exchange Type | Strong CatIon Exchanger |
Anion Exchange Capacity | 0.18 to 0.25 mM Hl+/mL |
Binding Capacity | 70 mg Ribonuclease A/mL Medium |
Product Type | SP Sepharose Fast Flow Media |
Flow Rate | 400 to 700 cm/Hoμr |
Particle Size | 45 to 165 μm |
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