MilliporeSigma™ Septin-3, Mouse, Unlabeled, Clone: 5C1.2,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN1530

Catalog No. MABN1530MI

Description

Neuronal-specific septin-3 (UniProt Q9UH03) is encoded by the SEPT3 (also known as SEP3) gene (Gene ID 55964) in human. Septins are GTPases capable of forming higher-order oligomers and filamentous polymers that contact with cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. Septins exist in complexes that are composed of ordered non-polar arrays of multiple different septins. Human genome contains 13 septin genes that are grouped into the SEPT2 subgroup (septins 1, 2, 4, and 5), the SEPT3 subgroup (septins 3, 9, and 12), the SEPT6 subgroup (septins 6, 8, 10, 11, and 14), and the SEPT7 subgroup (septin 7). All subgroups contain the well conserved GTP-binding domain and differ mainly in their C-terminal region, where coiled-coil sequences are found in SEPT2, SEPT6, and SEPT7, but not SEPT3, subgroups. Septin-3 is the only septin to be expressed almost exclusively in brain tissue, where it is enriched in nerve terminals, suggesting a role in synaptic vesicle function. Increased septin-3 expression is reported in several brain tumours and SEPT3 polymorphisms are linked to Alzheimer's disease. PKG-1 is shown to phosphorylate septin-3 in vitro, non-phosphorylated septin-3 localizes to the peripheral membrane, whereas the phosphorylated form is found in the cytosol. Murine and rat septin-3 is reported to form complexes with septin-5 and septin-7.

Specifications

• Antigen: Septin-3
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q9UH03
• Immunogen: KLH-conjugatged linear peptide coresponding to an internal sequence from the N-terminal half of human septin-3.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_663786
• Target Species: Human
• Form: Purified

• Applications: Western Blot
• Clone: 5C1.2
• Conjugate: Unconjugated
• Gene: SEPT3, SEP3
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 5C1.2 targets an internal epitope present in human spliced isoforms 1&2 (SEP3A and SEP3B), both mouse and all three rat spliced isoforms (G-septin-alpha, -beta, and -gamma) reporeted by UniProt (Q9UH03, Q9Z1S5, and Q9WU34, respectively). Target epitope is absent from human spliced isoform 3 (SEP3C).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.