Invitrogen™ SERPINA12 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530989

Catalog No. PIPA530989

Description

Recommended positive controls: 293T, A431, Jurkat, Raji. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis of vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members. The serpins are irreversible ('suicidal') serine-protease inhibitors, characterized by having more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure, which contains an exposed reactive center loop that acts as a pseudo-substrate for the target proteinase. Members of this family play an important role in a number of fundamental biological processes, including blood coagulation, fibrinolysis, complement activation, angiogenesis, inflammation, and tumor suppression. In humans, the serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin. Yet, its protease inhibitory activity is still unknown. Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. Administration of vaspin to obese mice improved glucose tolerance and insulin sensitivity, reflected by normalized blood glucose levels. It also led to the reversal of altered expression of diabetes-relevant adipocytokines, including leptin, adiponectin, resistin, and TNF-alpha. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the diabetic/obesity metabolic syndrome.

Specifications

• Antigen: SERPINA12
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SERPINA12
• Gene Alias: 4632419J12Rik; a12; Ol64; Ol-64; serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serine (or cysteine) peptidase inhibitor, clade A, member 12; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serpin A12; serpin family A member 12; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12; serpin peptidase inhibitor, clade A, member 12; Serpina12; Vaspin; visceral adipose tissue-derived serine protease inhibitor; visceral adipose tissue-derived serpin; visceral adipose-specific serpin
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 145264
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.57 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8IW75
• Gene Symbols: SERPINA12
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SERPINA12. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov