SerpinB9 Antibody (7D8) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NB017568	Novus Biologicals NB100643590.025MG	0.025 mg
NB10064359	Novus Biologicals NB10064359	0.1 mg

Description

SerpinB9 Monoclonal specifically detects SerpinB9 in Human, Mouse (Negative), Porcine (Negative) samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: SerpinB9
• Concentration: 1.0 mg/mL
• Applications: Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Flow Cytometry
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Apoptosis, Immunology
• Gene Accession No.: P50453
• Gene ID (Entrez): 5272
• Immunogen: Recombinant PI-9 produced in P. pastoris.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 7D8
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:100, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:50, Immunohistochemistry-Frozen 1:10-1:500
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse (Negative), Porcine (Negative), Human
• Gene Alias: CAP-3, CAP3PI-9, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, PI9protease inhibitor 9 (ovalbumin type), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9, serpin B9, serpin peptidase inhibitor, clade B (ovalbumin), member 9, serpin peptidase inhibitor, clade B, member 9
• Gene Symbols: SERPINB9
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: NB100-64359 is specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone 7D8 detects a band of approximately 42kDa under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence (2).

For Research Use Only