Invitrogen™ SETD2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534935

Catalog No. PIPA534935

Description

Recommended positive controls: 293T, Jurkat. Predicted reactivity: Rat (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.

Specifications

• Antigen: SETD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Setd2
• Gene Alias: 4921524K10Rik; BC031601; FLJ16420; FLJ22472; FLJ23184; FLJ45883; HBP231; HIF1; HIF-1; HIP-1; histone-lysine N-methyltransferase SETD2; hSET2; HSPC069; huntingtin interacting protein 1; huntingtin yeast partner B; Huntingtin-interacting protein 1; Huntingtin-interacting protein B; HYPB; Kiaa1732; Kif9; kinesin family member 9; KMT3A; LLS; lysine N-methyltransferase 3A; p231HBP; Protein-lysine N-methyltransferase SETD2; SET domain containing 2; SET domain containing 2, histone lysine methyltransferase; SET domain-containing protein 2; SET2; Setd2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29072
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9BYW2
• Gene Symbols: Setd2
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human HYPB/SETD2. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov