SETDB1, Mouse anti-Mouse, Clone: F0838, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABE106625	MilliporeSigma MABE106625UG	25 μg
MABE1066100	MilliporeSigma MABE1066100UG	100 μg

Description

Anti-SETDB1, clone F0838, Cat. No. MABE1066, is a highly specific mouse monoclonal antibody that detects SETDB1 and has been tested for use in Western Blotting.

Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in human. SETDB1 is ubiquitously expressed, but a strong expression is observed in liver and testis. It is a histone methyltransferase that specifically trimethylates Histone 3 Lysine 9 (H3K9). H3K9 trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB1 is reported to inhibit adipogenesis. It has also been shown that H3K4/H3K9me3 bivalent chromatin signature keeps expression of Cebpa and Pparg genes very low. Depletion of SETDB1 can induce adipogenesis in mesenchymal stem cells and in preadipocytes. SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and one MBD domain (aa 611-682). It also has a SET domain (aa 820-1282), a pre-SET (aa 744-817), and a post-SET domain (aa 1291-1307). The pre-SET, SET and post-SET domains are required for its methyltransferase activity. In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster. (Ref.: Matsumura, Y., et al., (2015), Mol. Cell 60(4); 584- 596).

Specifications

• Antigen: SETDB1
• Dilution: Western Blotting Analysis: A representative lot detected SETDB1 in Western Blotting applications (Matsumura, Y, et. al. (2015). Mol Cell. 60(4):584-96).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Gene Alias: Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;ESET;SET domain bifurcated 1
• Immunogen: His-tagged recombinant fragment corresponding to 213 amino acids from the N-terminal half of murine Histone-lysine N-methyltransferase SETDB1.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.

• Clone: F0838
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Epigenetics & Nuclear Function
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
• Gene Symbols: Setdb1;Eset;Kiaa0067
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: Clone F0838 recognizes SETDB1 in murine embryonic stem cells. It targets an epitope with in 213 amino acids from the N-terminal half of isoform 1 and 7.