Invitrogen™ SHANK1/SHANK3 Monoclonal Antibody (N367/51), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545673

Catalog No. PIMA545673

Description

Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2. 1 μg/mL of MA5-45673 was sufficient for detection of SHANK1/SHANK3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 190kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2. This antibody was formerly sold as clone S367-51.

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation. {PubMed:21606927, PubMed:21795692, PubMed:23100419, PubMed:23897824, PubMed:24089484}.

Specifications

• Antigen: SHANK1/SHANK3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: D3YZU1, Q4ACU6, Q9BYB0, Q9JLU4, Q9WV48, Q9Y566
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 243961, 50944, 58234, 59312, 78957, 85358
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: N367/51
• Conjugate: APC
• Gene: Shank1
• Gene Symbols: Shank1, Shank3
• Immunogen: Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov