Invitrogen™ SHARPIN Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA588439

Catalog No. PIPA588439

Description

Positive Samples: 293T, LO2, HeLa, A-549, Rat brain; Cellular Location: Cell junction, Cytoplasm, cytosol, synapse Immunogen sequence: MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS LHFLNPQEAQ RWAVLVRGAT VEGQNG.

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

Specifications

• Antigen: SHARPIN
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SHARPIN
• Gene Alias: 0610041B22Rik; AW121341; Conneck1; cpdm; hSIPL1; mSIPL1; protein kinase C-interacting protein RBCC like 1; PSEC0216; RBCKL1; SHANK associated RH domain interactor; SHANK-associated RH domain interacting protein; SHANK-associated RH domain interactor; shank-associated RH domain-interacting protein; shank-interacting protein; shank-interacting protein-like 1; SHARPIN; SIPL1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 106025, 81858, 81859
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.08 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: Q91WA6, Q9EQL9, Q9H0F6
• Gene Symbols: SHARPIN
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human SHARPIN (NP_1122363?).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG