Invitrogen™ SHH Monoclonal Antibody (G.162.10)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA514909

Catalog No. PIMA514909

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with transfected IHH and DHH.

Sonic Hedgehog (SHH), which is expressed only during embryogenesis, is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the Sonic Hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. In addition, it is thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.

Specifications

• Antigen: SHH
• Classification: Monoclonal
• Concentration: 52 μg/mL
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: Q15465, Q63673
• Gene Symbols: Shh
• Immunogen: Synthetic peptide corresponding to residues surrounding Glu53 of human Shh.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: G.162.10
• Conjugate: Unconjugated
• Gene: Shh
• Gene Alias: 9530036O11Rik; Dsh; fc83d08; hedgehog; hemimelic extra toes; Hhg1; HHG-1; HLP3; HPE3; Hx; Hxl3; M100081; MCOPCB5; morphogen; shh; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; shh.L; shha; ShhN; ShhNC; ShhNp; short digits; SMMCI; sonic hedgehog; sonic hedgehog a; sonic hedgehog homolog; sonic hedgehog L homeolog; sonic hedgehog protein; Sonic hedgehog protein 19 kDa product; Sonic hedgehog protein A; Sonic hedgehog protein A N-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; sonic hedgehog signaling molecule a; sonic-you; syu; TPT; TPTPS; vhh1; vhh-1; wu:fc83d08; XELAEV_18030676mg; Xhh; xshh; X-SHH
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29499, 6469
• Content And Storage: -20°C
• Form: Liquid