Invitrogen™ Shiga toxin 2A Monoclonal Antibody (B19J)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA17101

Catalog No. MA17101

Description

MA1-7101 detects the Verotoxin. This antibody is specific for Verotoxin II - A subunit (SLT-2a). MA1-7101 has been successfully used in ELISA procedures. The MA1-7101 immunogen is purified native toxoid.

Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share 99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.

Specifications

• Antigen: Shiga toxin 2A
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Formulation: PBS with 0.1% sodium azide; pH 7.2
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG2

• Applications: ELISA
• Clone: B19J
• Conjugate: Unconjugated
• Gene Alias: Shiga toxin; Shigatoxin Type 2; Shigatoxin Type II; STX2; STX-2a; STX-IIa
• Immunogen: Purified native toxoid.
• Quantity: 1 mL
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Form: Liquid