Novus Biologicals™ Shiga toxin subunit B Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for E. coli Shiga toxin subunit B.

Specifications

• Assay Range: 34.38 pg/mL
• Assay Sensitivity: 34.38-2200 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for E. coli Shiga toxin subunit B.
• Target Species: E. coli
• For Use With (Application): Sandwich ELISA
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Shiga toxin subunit B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Shiga toxin subunit B monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 50 ng of recombinant Shiga toxin subunit B.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Shiga toxin subunit B
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in dilution buffer before use. 3. Standard: Reconstitute with 1 ml dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in dilution buffer, and a high standard of 2200 pg/mL is recommended.