DescriptionThis solid phase sandwich ELISA utilizes a monoclonal antibody specific for E. coli Shiga toxin subunit B.
|Shiga toxin subunit B|
|This solid phase sandwich ELISA utilizes a monoclonal antibody specific for E. coli Shiga toxin subunit B.|
|Storage is content dependent.|
|1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in dilution buffer before use. 3. Standard: Reconstitute with 1 ml dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in dilution buffer, and a high standard of 2200 pg/mL is recommended.|
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