Invitrogen™ SIAH2 Monoclonal Antibody (24E6H3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524780

Catalog No. PIMA524780

Description

This is specific for human SIAH2 and Drosophila SINA in proliferating cells and stem cells. This does not detect Drosophila SINAH or normal, nondividing cells.

SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1 alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.

Specifications

• Antigen: SIAH2
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: tissue culture supernatant with 0.1% sodium azide
• Gene Accession No.: O43255, P21461
• Gene Symbols: SIAH2, sina
• Immunogen: Synthetic peptide made to an N-terminal portion of the drosophila SIAH2 protein (within residues 1-100).
• Regulatory Status: RUO
• Gene ID (Entrez): 100622531, 39884, 6478
• Content And Storage: 4°C, do not freeze
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 24E6H3
• Conjugate: Unconjugated
• Gene: SIAH2
• Gene Alias: AA415433; E3 ubiquitin-protein ligase SIAH2; EC 6.3.2; hSiah2; mSiah2; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 2; seven in absentia homolog 2; siah E3 ubiquitin protein ligase 2; SIAH2; siah-2; Sinh2
• Host Species: Mouse
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Pig, Drosophila
• Product Type: Antibody
• Isotype: IgG1