Siglec-1/CD169 Antibody (ED3) - BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB100653010.025MG

Catalog No. NB017705

Description

Siglec-1/CD169 Monoclonal specifically detects Siglec-1/CD169 in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen.

Specifications

• Antigen: Siglec-1/CD169
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Dilution: Flow Cytometry 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:50-1:250
• Gene Alias: CD169, FLJ00051, sialic acid binding Ig-like lectin 1, sialoadhesin, sialoadhesin
• Host Species: Mouse
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Gene ID (Entrez): 6614
• Target Species: Rat
• Form: Purified

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
• Clone: ED3
• Conjugate: Unconjugated
• Formulation: PBS (pH 7.4) with 0.09% Sodium Azide
• Gene Symbols: SIGLEC1
• Immunogen: Rat Spleen cell homogenate
• Quantity: 0.025 mg
• Primary or Secondary: Primary
• Test Specificity: NB100-65301 recognizes the rat CD169 cell surface antigen, a 185kD molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2a

For Research Use Only