Promega SignaTECT™ Protein Kinase Assay Systems

Description

Contain proprietary SAM2 Biotin Capture Membrane for higher binding capacity and specificity

• Produces lower backgrounds and higher signal-to-noise ratios as compared to traditional P81 phosphocellulose methods
• Perforated and numbered membrane allows measurement of up to 96 kinase reactions; ideal for kinetic studies
• Assay requires little “hands-on” manipulation and is complete in under an hour
• Can be used in a variety of buffer/reaction conditions including cell extracts
• Each assay system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as necessary reaction components
• PTK assay system also contains two proprietary peptide substrates

User must supply [γ-32^P] ATP

U.S. Patents 6,066,462 and 6,348,310 and other patents pending.

Specifications

• Target: Calcium/Calmodulin
• For Use With (Application): Can be used in a variety of Buffer or reaction conditions including cell extracts
• Includes: ATP, BSA, SAM2 Biotin capture membrane, Termination Buffer, CaM KII Biotinylated Peptide Substrate, reaction control, activation Buffer
• Content And Storage: -20°C
• Product Type: Protein Kinase Assay
• Quantity: 96 Reactions