SIRPA Mouse anti-Human, Clone: OTI7B3, lyophilized, TrueMAB™

Mouse Monoclonal Antibody

Supplier:  OriGene CF807751

Catalog No. 50-166-8460

Description

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product No. 7KMWCO

SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.

Specifications

• Antigen: SIRPA
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: SIRPA
• Gene Alias: BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS1, SIRP
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 140885
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: OTI7B3
• Formulation: PBS with 8% trehalose and no preservative; pH 7.3
• Gene Accession No.: P78324
• Gene Symbols: SIRPA
• Immunogen: Full length human recombit protein of human SIRPA produced in HEK293T cell.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1