Invitrogen™ SKA1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520817

Catalog No. PIPA520817

Description

A suggested positive control is A549 cell lysate. PA5-20817 can be used with blocking peptide PEP-0931.

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.

Specifications

• Antigen: SKA1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SKA1
• Gene Alias: 2810433K01Rik; AV117428; C18orf24; RGD1310784; SKA1; spindle and kinetochore associated complex subunit 1; spindle and kinetochore-associated protein 1; spindle and KT (kinetochore) associated 1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 220134, 291441, 66468
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: B0BN28, Q96BD8, Q9CPV1
• Gene Symbols: SKA1
• Immunogen: A 13 amino acid peptide from near the carboxy terminus of human SKA1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG