Invitrogen™ SKA3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA558722

Catalog No. PIPA558722

Description

Immunogen sequence: NSVHEQEAIN SDPELSNCEN FQKTDVKDDL SDPPVASSCI SEKSPRSPQL SDFGLERYIV SQVLPNPPQA VNNYKEEPVI VTPPTKQ Highest antigen sequence identity to the following orthologs: Mouse - 54%, Rat - 44%.

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.

Specifications

• Antigen: SKA3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SKA3
• Gene Alias: C13orf3; F630043A04Rik; RAMA1; RGD1307201; RP11-101P17.12-002; Ska3; spindle and kinetochore associated complex subunit 3; spindle and kinetochore-associated protein 3
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 221150
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.55 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q8IX90
• Gene Symbols: SKA3
• Immunogen: Recombinant protein corresponding to Human SKA3. Recombinant protein control fragment (Product #RP-97983).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG