SKA3 Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A304215A

Catalog No. 50-156-7147

Description

The recommended shelf life for this product is 1 year from date of receipt.

Upon entry into mitosis, the cell′s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintece of chromosome cohesion in mitosis.

Specifications

• Antigen: SKA3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SKA3
• Gene Alias: RP11-101P17.12-002, C13orf3, RAMA1; spindle and kinetochore-associated protein 3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 221150
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: Q8IX90
• Gene Symbols: SKA3
• Immunogen: Between 362 and 412
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG