SAP (SLAM-Associated Protein) Mouse anti-Human, Clone: 10C4.2, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 14988882
Description: This 10C4.2 monoclonal antibody reacts with human SLAM-associated protein (SAP). Upon SLAM receptor ligation, this 16-kDa SH2 domain-containing adaptor protein recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events. SAP is expressed in T, NK, and NKT cells, as well as eosinophils and platelets. This protein has also been detected in B cells and tumor cell lines such as Jurkat and Raji. Studies demonstrate that SAP expression in T follicular helper cells is required for germinal center formation. Moreover, this protein is involved in NKT cell development, Th1/Th2 differentiation, and controlling NK cytotoxicity. The role of SAP in B cells, however, is currently under investigation. Finally, deletion of the gene encoding SAP, SH2D1A, results in X-linked lymphoproliferative disease in humans. Applications Reported: This 10C4-SAP (10C4.2) antibody has been reported for use in immunoblotting and immunocytochemistry (ICC). Applications Tested: This 10C4.2 antibody has been tested by immunoblotting of reduced cell lysate prepared from Jurkat cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and maligt lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene.
|SAP (SLAM-Associated Protein)|
|PBS with 0.09% sodium azide; pH 7.2|
|DSHP, EBVS, IMD5, LYP, MTCP1, SH2D1A, XLP, XLPD|
|Immunocytochemistry, Immunofluorescence, Western Blot|
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