SLAMF7 (CD319), Mouse anti-Human, Clone: 162, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABF209325UG

Catalog No. MABF209325

Description

Anti-SLAMF7 (CD319), clone 162, Cat. No. MABF2093, is a mouse monoclonal antibody that detects SLAM family member 7 and has been tested for use in Flow Cytometry, Function Analysis, Immunohistochemistry (Paraffin), Immunoprecipitation, and Inhibition studies.

SLAM family member 7 (UniProt: Q9NQ25; also known as SLAMF7, CD2 subset 1, CD2-like receptor-activating cytotoxic cells, CRACC, Membrane protein FOAP-12, Novel Ly9, Protein 19A, CD319) is encoded by the SLAMF7 (also known as CS1, UNQ576/PRO1138) gene (Gene ID: 57823) in human. SLAMF-7 is a single-pass type I membrane protein that is expressed in spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung and trachea. Expression has also been detected in multiple myeloma cells, NK cells, activated B-cells, and NK-cell line, but not in promyelocytic, B-, or T-cell lines. It serves as a receptor on immune cells, including natural killer (NK) cells. SLAMF7 is reported to mediate both activating and inhibitory effects on NK cells, depending on the expression of adaptor EAT-2. In cells lacking EAT-2 it mediates inhibition via SHIP-1, which is recruited via Tyr 261 of SLAMF7. SLAMF7 is shown to be involved in phagocytosis of hematopoietic tumor cells independent of signaling lymphocyte activation molecule-associated protein (SAP) adaptors, but depends on its ability to interact with integrin Mac-1 and utilizes signals involving ITSM motif (aa 302-307). SLAMF7 is synthesized with a signal peptide (aa 1-22), which is subsequently removed to generate the mature form that has an extracellular domain (aa 23-226), a short transmembrane domain (aa 227-247), and a cytoplasmic tail (aa 248-335). Seven different isoforms of SLAMF7 have been described that are produced by alternative splicing. Clone 162 is shown to block the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to Anti-CD47 antibodies. (Ref.: Guo, H., et al. (2015). Mol. Cell. Biol. 35 (1); 41-51; Chen, J., et al. (2017). Nature 544 (7651); 493-497).

Specifications

• Antigen: SLAMF7 (CD319)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2b in PBS without azide.
• Gene Symbols: SLAMF7;CS1;UNQ576/PRO1138
• Immunogen: Recombinant protein fragment corresponding to 204 amino acids from the extracellular domain of human SLAM family member 7 fused with human IgG1 constant region.
• Quantity: 25 μg
• Research Discipline: Inflammation & Immunology
• Gene ID (Entrez): NP_067004.3
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Inhibition Assays
• Clone: 162
• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected SLAMF7 (CD319) in human bone marrow tissue sections.Flow Cytometry Analysis: A representative lot detected SLAMF7 (CD319) in Flow Cytometry applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21).Affects Function Analysis: A representative lot stimulated SLAMF7, which resulted in an increase SHIP-1 tyrosine phosphorylation in OPM2 and MM1S cells expressing EGFR-CD45. (Guo, H., et. al. (2015). Mol Cell Biol. 35(1):41-51).Inhibition Analysis: A representative lot blocked the augmented capacity of human blood-derived macrophages to engulf Raji cells in response to anti-CD47 antibodies. (Chen, J., et. al. (2017). Nature. 544(7651):493-497).Immunoprecipitation Analysis: A representative lot immunoprecipitated SLAMF7 (CD319) in Immunopreciptation applications (Bouchon, A., et. al. (2001). J Immunol. 167(10):5517-21; Tassi, I., et. al. (2005). J Immunol. 175(12):7996-8002).
• Gene Alias: SLAM family member 7;CD2 subset 1;CD2-like receptor-activating cytotoxic cells;CRACC;Membrane protein FOAP-12;Novel Ly9;Protein 19A;CD319
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 162 is a mouse monoclonal antibody that detects SLAM family member 7 (SLAMF7) in human cells. It targets an epitope with in the extracellular domain.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG2b κ