Thermo Scientific™ Slide-A-Lyzer™ Dialysis Flasks, 10K MWCO, 250 mL, 4 Flasks PROMO

Description

Facilitate removal of salts and small contaminants from proteins or macromolecules in sample volumes up to 250 mL. Each Slide-A-Lyzer Dialysis Flask provides flotation disks that when attached to the neck of the flask, allows the flask to float in a vertical position for simple and safe handling.

The dialysis flasks are available in distinct colors, corresponding to the pore size (MWCO) of the dialysis membrane: purple (20,000 Daltons), orange (10,000 Daltons), pink (3,500 Daltons), and blue (2,000 Daltons).

Slide-A-Lyzer Dialysis Flasks are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of an inert plastic body. The membrane and flask materials are compatible with most laboratory buffers.

Features of Slide-A-Lyzer Dialysis Flasks:

• Easy to use—simply pipette or pour sample into flask and begin dialysis
• Fast dialysis—flat flask chamber with two membranes provides high surface area to volume ratio, enabling dialysis of a 250 mL sample in 8 hours to overnight
• High recovery—rectangular flask design maximizes recovery of entire sample volume via opening at top of flask
• Multiple molecular-weight cut offs—select the membrane MWCO that best suits your sample's molecular weight

Dialysis membrane specifications:

• Composition: regenerated-cellulose
• Thickness: 0.9–1.2 mil (22.5 to 30 μm); 2K MWCO = 2 mil (50 μm)
• Hydration time required before use: 2 minutes
• Glycerol content: trace (10K MWCO content is 21%)
• Sulfur content: 0.04–0.17%
• Heavy metals content: trace

With Slide-A-Lyzer Dialysis Flasks, typical low-molecular weight contaminant removal, buffer exchange, and desalting can be accomplished in eight hours to overnight. The rate of dialysis depends on a variety of factors, including sample volume, size and shape of the molecule being dialyzed, agitation, and temperature. Minimum dialysis time must be determined empirically for each specific system.

Specifications

• Content And Storage: Shipped at ambient temperature. Upon receipt, store at room temperature.
• Purification Target: Buffer Exchange, Protein
• Membrane: Cellulose Membrane, Dialysis
• Format: Flask
• MWCO: 10 kDa
• Product Line: Slide-A-Lyzer™
• Quantity: 4 Flasks
• Sample Volume (Metric): 250 mL