Invitrogen™ SLP76 Monoclonal Antibody (SLP-76/03)

Catalog No.	Quantity
01-676-125	100 μg

Catalog No. 01-676-125 Supplier Invitrogen™ Supplier No. MA119369

Description

Mouse Monoclonal Antibody

This antibody reacts with SLP76, a 76kDa cytosolic adaptor protein that is involved in signaling of various hematopoietic cells, such as T cells, mast cells or neutrophils; in B cells, however, it is replaced by SLP65.

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Specifications

• Antigen: SLP76
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: SLP-76/03
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene: LCP2
• Gene Accession No.: Q13094, Q60787
• Gene Alias: 76 kDa tyrosine phosphoprotein; AI323664; BB161688; Lcp2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP76; SLP-76; SLP-76 tyrosine phosphoprotein; twm
• Gene Symbols: LCP2
• Host Species: Mouse
• Immunogen: Bacterially expressed fusion protein representing amino acids 216-434 of human SLP76 with histidine tag.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100511843, 16822, 3937
• Target Species: Human, Mouse, Pig
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b