Smad1, p Ser206 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP177860

Catalog No. NBP177860

Description

Smad1 Polyclonal specifically detects Smad1 in Human samples. It is validated for Western Blot, ELISA, Immunoprecipitation.

Specifications

• Antigen: Smad1 (p Ser206)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, pSMAD, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
• Host Species: Rabbit
• Molecular Weight of Antigen: 52.26 kDa
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad1 protein at the pS206 residue. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with Smad1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.

• Applications: Western Blot, ELISA, Immunoprecipitation
• Concentration: 1.31 mg/ml
• Dilution: Western Blot 1:500, ELISA 1:25000-1:30000, Immunoprecipitation 2 ug
• Gene Accession No.: Q15797
• Gene Symbols: SMAD1
• Immunogen: This affinity purified Smad1 [p Ser206] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human Smad1 protein. (Uniprot: Q15797)
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4086
• Target Species: Human, Mouse, Rat, Canine
• Isotype: IgG

For Research Use Only