SMAD2 Rabbit anti-Human, Mouse, Rat, Polyclonal, Proteintech

Rabbit Polyclonal Antibody

Supplier:  Proteintech Group Inc 125701AP150UL

Catalog No. 50-172-8076

Description

This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa.

SMAD2 (MADH2, MAD2) regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 interacts with the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal regulated kinase 1 (ERK1) enhances SMAD2 transcriptional activity, which is negatively regulated by calmodulin. In response to the TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the SMAD2 protein have been observed.

Specifications

• Antigen: SMAD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SMAD2
• Gene Alias: hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17126, 29357, 4087
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 0.22 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O70436, Q15796, Q62432
• Gene Symbols: Smad2
• Immunogen: SMAD2 Fusion Protein Ag3237
• Quantity: 150 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG