Abnova™ SMAD3 HEK293 Cell Transient Overexpression Lysate (Nondenatured)

Description

Human overexpression lysate

Specifications

• Cell Line: HEK293
• For Use With (Application): Immunoprecipitation, Western Blot
• Description: SMAD3 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
• Gene Accession No.: NM_005902
• Lysis Buffer: Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. ;Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
• Molecular Weight (g/mol): Theoretical M.W. 48kDa
• Plasmid: pCMV-SMAD3 full length
• Quality Control Testing: Transient overexpression cell lysate was tested with anti-SMAD3 antibody (Mfr. No. H00004088-M01) by Western Blot.
• Quantity: 100 μg
• Storage Buffer: In modified RIPA Lysis Buffer.
• Host Species: Human
• Concentration: 2 mg/mL
• Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.