Smad3, p Ser423, p Ser425 Antibody, PE, Novus Biologicals™

Rabbit Polyclonal Antibody

Supplier:  Novus Biologicals NBP177836PE

Catalog No. NBP177836PE

Description

Smad3 (p Ser423) Polyclonal antibody specifically detects Smad3 (p Ser423) in Human, Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: Smad3 (p Ser423)
• Classification: Polyclonal
• Dilution: Flow Cytometry
• Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
• Host Species: Rabbit
• Purification Method: Affinity Purified
• Research Discipline: Phospho Specific, Signal Transduction, Wnt Signaling Pathway
• Gene ID (Entrez): 4088
• Target Species: Human, Mouse, Rat, Pig, Bovine, Chicken, Xenopus species, Zebrafish
• Isotype: IgG

• Applications: Flow Cytometry
• Conjugate: PE
• Formulation: PBS with 0.05% Sodium Azide
• Gene Symbols: SMAD3
• Immunogen: Smad3 [p Ser423, p Ser425] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein. (Uniprot: P84022)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus species laevis, Xenopus species tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
• Content And Storage: Store at 4C in the dark.