Invitrogen™ SMAD3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA534774

Catalog No. PIPA534774

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, rat brain, SMAD2 and SMAD3-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (95%), Dog (100%), Pig (100%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Specifications

• Antigen: SMAD3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Smad3
• Gene Alias: AU022421; DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MAD (mothers against decapentaplegic, Drosophila) homolog 3; MAD homolog 3; MAD homolog 3a; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; mad3; MAD-3; madh3; madh3a; madh3-A; MGC60396; mMad3; mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3a; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD; Smad 3; SMAD family member 3; SMAD family member 3 L homeolog; SMAD family member 3a; SMAD, mothers against DPP homolog 3; Smad3; smad3.L; smad3a; TGF beta response effector Smad3; TGF-beta response effector Smad3; transcription factor; transcription factor Smad3; wu:fa99e03; XELAEV_18018454mg; XenMLP; Xmad3; XSmad3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17127, 25631, 4088
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.75 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P84022, P84025, Q8BUN5
• Gene Symbols: Smad3
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SMAD2/SMAD3. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG