SMAD4 Rabbit anti-Human, Mouse, Polyclonal, Proteintech

Rabbit Polyclonal Antibody

Manufacturer:  Proteintech Group Inc 102311AP150UL

Catalog No. 50-172-6133

Description

This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Specifications

• Antigen: SMAD4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SMAD4
• Gene Alias: DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17128, 4089
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 0.24 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.3
• Gene Accession No.: P97471, Q13485
• Gene Symbols: Smad4
• Immunogen: SMAD4 Fusion Protein Ag0299
• Quantity: 150 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Liquid