Invitrogen™ SMAD4 Recombinant Rabbit Monoclonal Antibody (RM277)

Catalog No.	Quantity
PIMA544588	100 μL

Catalog No. PIMA544588

Supplier: Invitrogen™ MA544588

Description

Rabbit Recombinant Monoclonal Antibody

This antibody reacts to 5-Carboxylcytosine in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.

Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-relatedprotein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

Specifications

• Antigen: SMAD4
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: RM277
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1% BSA, 50% glycerol and 0.09% sodium azide
• Gene: SMAD4
• Gene Accession No.: O70437, P97471, Q13485
• Gene Alias: AW743858; D18Wsu70e; Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; DPC4; hSMAD4; JIP; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Madh4; mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; MYHRS; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; SMAD4
• Gene Symbols: SMAD4
• Host Species: Rabbit
• Immunogen: A peptide corresponding to the C-terminus of human Smad4.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17128, 4089, 50554
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG