Invitrogen™ SMC5 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530460

Catalog No. PIPA530460

Description

Recommended positive controls: NT2D1. Predicted reactivity: Mouse (80%), Rat (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.

Specifications

• Antigen: SMC5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Smc5
• Gene Alias: hSMC5; KIAA0594; mKIAA0594; MLZ-453; mSMC5; Protein expressed in male leptotene and zygotene spermatocytes 453; SMC protein 5; Smc5; SMC-5; SMC5 structural maintenance of chromosomes 5-like 1; Smc5l1; structural maintenance of chromosomes 5; structural maintenance of chromosomes protein 5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 226026, 23137
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8CG46, Q8IY18
• Gene Symbols: Smc5
• Immunogen: Recombinant fragment corresponding to a region within amino acids 257 and 493 of Human SMC5.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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