Invitrogen™ Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), Alexa Fluor™ 488, eBioscience™

Catalog No.	Quantity
53-640-082	100 μg

Catalog No. 53-640-082 Supplier Invitrogen™ Supplier No. 53640082

Description

Mouse Monoclonal Antibody

Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells. Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells. In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining. In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer. This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains. Applications Reported: This SMMS-1 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This SMMS-1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 30 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

Specifications

• Antigen: Smooth Muscle Myosin
• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
• Classification: Monoclonal
• Clone: SMMS-1
• Concentration: 0.5 mg/mL
• Conjugate: Alexa Fluor 488
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: MYH11
• Gene Accession No.: P35749, Q63862
• Gene Alias: AAT4; AV071570; contractile protein; FAA4; KIAA0866; LOW QUALITY PROTEIN: myosin-11; Myh11; myh11 {ECO:0000312; Myosin heavy chain 11; myosin heavy chain 11, smooth muscle; myosin heavy chain 21; myosin heavy chain isoform SMA; myosin heavy chain SMA; myosin heavy chain, smooth muscle isoform; myosin, heavy chain 11, smooth muscle; myosin, heavy polypeptide 11, smooth muscle; myosin-11; RGD:3136}; SM1; SM2; SMHC; SMMHC; smooth muscle myosin heavy chain
• Gene Symbols: MYH11
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 101097693, 24582, 4629, 479836, 530050
• Target Species: Bovine, Canine, Feline, Human, Rat
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ