Invitrogen™ SMURF1 Monoclonal Antibody (1A9H4)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA550289

Catalog No. FEMA550289

Description

SMURF1 Monoclonal Antibody for Western Blot, ICC/IF, IHC (P), Flow, ELISA

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e. g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Specifications

• Antigen: SMURF1
• Classification: Monoclonal
• Concentration: 0.95 mg/mL
• Formulation: PBS with 50% glycerol and 0.03% ProClin 300, 0.1% sodium azide; pH 7.3
• Gene Accession No.: Q9HCE7
• Gene Symbols: Smurf1
• Immunogen: Recombinant Human E3 ubiquitin-protein ligase SMURF1 protein (198-374AA).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 1A9H4
• Conjugate: Unconjugated
• Gene: Smurf1
• Gene Alias: 4930431E10Rik; E3 ubiquitin ligase SMURF1; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF1; E3 ubiquitin-protein ligase SMURF2; HECT-type E3 ubiquitin transferase SMURF1; HECT-type E3 ubiquitin transferase SMURF2; hSMURF1; hSMURF2; KIAA1625; mKIAA1625; RGD1309707; SMAD specific E3 ubiquitin protein ligase 1; SMAD specific E3 ubiquitin protein ligase 2; Smad ubiquitination regulatory factor 1; SMAD ubiquitination regulatory factor 2; Smad-specific E3 ubiquitin ligase 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF1; SMURF2
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 57154
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid