Invitrogen™ SP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595583

Catalog No. PIPA595583

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Jurkat whole cell, human A431 whole cell, human 293T whole cell, human Hela whole cell. IHC: human non-samll cell lung carcinoma tissue, human ovarian cancer tissue, mouse lung tissue, rat lung tissue. ICC/IF: A549 cell. Flow: A431 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Specifications

• Antigen: SP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: SP1
• Gene Alias: 1110003E12Rik; AA450830; AI845540; HGNC:11205; SP1; Sp1 transcription factor; Sp1-1; specificity protein 1; trans-acting transcription factor 1; transcription factor Sp1; TSFP1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20683, 24790, 6667
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: O89090, P08047, Q01714
• Gene Symbols: SP1
• Immunogen: E.coli-derived human SP1 recombinant protein (Position: Q384-A603).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov