Invitrogen™ SPARC Monoclonal Antibody (ON1-1)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 335500

Catalog No. 33-550-0

Description

Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with bovine bone osteonectin (SPARC). The monoclonal antibody was harvested from ascitic fluid. Specificity: This antibody reacts with both bone derived and platelet derived Osteonectin (ELISA). This antibody does not react with non-activated platelet and reacts with thrombin-stimulated platelet (flow cytometric analysis). This antibody reacts with human and bovine Osteonectin (Western blot analysis). This antibody cross reacts with rabbit and porcine Osteonectin. (ELISA) Storage: 4°C. This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Reconstitution: Dissolve the lyophilized antibody in 50 μL of distilled water (final concentration: 2.0 mg/mL). This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 μL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution shoul...

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: SPARC
• Classification: Monoclonal
• Concentration: 2.0 mg/mL
• Formulation: PBS with 1% BSA and no preservative; pH 7.4
• Gene Accession No.: P09486, P13213, P20112, P36233
• Gene Symbols: SPARC
• Immunogen: Full-length Bovine bone osteonectin (SPARC).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rabbit, Bovine, Pig
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
• Clone: ON1-1
• Conjugate: Unconjugated
• Gene: SPARC
• Gene Alias: Basement membrane protein 40; basement-membrane protein 40; BM40; BM-40; Cysteine rich protein; OI17; ON; Osteonectin; osteonectin precursor; Secreted acidic cystein rich glycoprotein; secreted acidic cysteine rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted protein acidic and cysteine rich; secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); secreted protein, acidic, cysteine-rich [osteonectin]; SPARC
• Host Species: Mouse
• Purification Method: Ion-exchange Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100009355, 282077, 595124, 6678
• Content And Storage: 4°C
• Form: Lyophilized