Thermo Scientific™ Spectra™ Multicolor High Range Protein Ladder

Description

A mixture of eight blue-, green- and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

Thermo Scientific™ Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green and orange-stained proteins (40 to 300kDa) for use as size standards for high MW proteins in gel electrophoresis and Western blotting.

This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Three different chromophores (blue, orange, green) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required.

Highlights:

• Size range – eight proteins spanning 40 to 300kDa

• Multicolor – three different colors for unambiguous band-size assignment

• Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

• Sharp bands – color-coded bands of similar intensity for easy visualization

• Quality tested – each lot evaluated by SDS-PAGE and Western blotting

• Membrane-compatible – colored bands transfer to membranes for Western blotting

Includes:

Dye-stained proteins in 62.5mM Tris-H³PO⁴ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN³ and 33% glycerol.

Recommended for:

Monitoring of protein migration during SDS-PAGE; Verifying Western transfer efficiency; Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots; Locating a protein of interest for excision from an unstained preparative gel

Specifications

• Content And Storage: Contents: two vials of 250 μL each
  
  Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol
  
  Storage: Upon receipt store at -20°C
  
  
• Detection Method: Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm)
• Number of Markers: 8
• Product Type: Protein Ladder
• Ready to Load: Yes
• Recommended Applications: Tris-acetate gels
• Size Range: 40 to 300 kDa
• Stain Type: 3 colors: Blue, Orange, Green
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Molecular Weight (g/mol): 300, 250, 180, 130, 100, 70, 50, 40 kDa
• Product Line: Spectra™
• Quantity: 2 x 250 μL
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• System Type: Western Blotting, SDS-PAGE

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.