Invitrogen™ Spectrin beta-4 Monoclonal Antibody (N393/2)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA527608

Catalog No. PIMA527608

Description

A 1:100 dilution of MA5-27608 was sufficient for detection of Beta 4 Spectrin in 20 μg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately >200kDa. Does not cross react with other Beta-spectrins This antibody was formerly sold as clone S393-2.

Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart. Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier.

Specifications

• Antigen: Spectrin beta-4
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.1% sodium azide; pH 7.4
• Gene Accession No.: Q9H254
• Gene Symbols: SPTBN4
• Immunogen: Fusion protein amino acids 1621-1832 (C-terminal repeats 14 to 15) of human Beta4-spectrin.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry
• Clone: N393/2
• Conjugate: Unconjugated
• Gene: SPTBN4
• Gene Alias: 1700022P15Rik; 5830426A08Rik; beta-IV spectrin; beta-spectrin 4; dyn; EMBL BAB83243.1; KIAA1642; lnd; lumbosacral neuroaxonal dystrophy; neuroaxonal dystrophy; nmf261; nmf379; quivering; qv; ROSA62; SpbIV; spectrin beta 4; spectrin beta chain, brain 3; spectrin beta chain, non-erythrocytic 4; spectrin beta, non-erythrocytic 4; spectrin, beta, non-erythrocytic 4; spectrin, non-erythroid beta chain 3; SPNB4; SPTBN3; SPTBN4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 308458, 57731, 80297
• Content And Storage: -20°C
• Form: Liquid