Invitrogen™ SPHK1 Monoclonal Antibody (M540)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA547736

Catalog No. PIMA547736

Description

The antibody detects 42 kDa* full-length recombinant human and mouse SK1 proteins, as well as detects 42, 50, and 60 kDa forms of SK1 in human HeLa cells.

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P). S1P signals through G-protein coupled receptors and controls diverse cellular processes. Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer. SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

Specifications

• Antigen: SPHK1
• Classification: Monoclonal
• Concentration: 2 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q8CI15, Q91V26, Q9NYA1
• Gene Symbols: SPHK1
• Immunogen: Clone M540 was generated from a SK1 synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal region of mouse SK1. This sequence has high homology to human and rat SK1, and is not homologous to sequences in SK2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody

• Applications: Western Blot, Immunocytochemistry
• Clone: M540
• Conjugate: Unconjugated
• Gene: SPHK1
• Gene Alias: 1110006G24Rik; Acetyltransferase SPHK1; RP23-193A16.4; SK 1; Sk1; SKase; Sphingosine kinase 1; SPHK; SPHK1; SPK; SPK 1; Spk1
• Host Species: Rat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 170897, 20698, 8877
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid